Coding

Part:BBa_K4343097

Designed by: Xiaolu Sun   Group: iGEM22_NNU-China   (2022-10-09)


EgDes5

EgDes5 encodes ∆-5 desaturase from Euglena gracilis which functions in the Δ-9 elongase/desaturase pathway and could convert C20:3 to C20:4.

Description

The EgDes5 gene encodes a ∆-5 desaturase of 449 amino acids from Euglena gracilis that catalyzes the conversion of dihomo-γ-linolenic acid (DGLA; C20: 3 n-6) to arachidonic acid (ARA; C20:4 n-6).


References

Pollak, D.W. et al. Isolation of a ∆5 desaturase gene from Euglena gracilis and functional dissection of its HPGG and HDASH motifs. Lipids 47, 913–926 (2012).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 42
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 963
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1367


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Categories
Parameters
None